The calibration process typically involves the following steps:
Preparation: Ensure the incubator is clean and has been running at the desired set points for at least 12 hours to stabilize conditions.
- Temperature Calibration:
Use a calibrated reference thermometer to measure the internal temperature.Compare the reading with the incubator’s display and adjust if discrepancies are found.
- CO₂ Calibration:
Utilize an independent CO₂ analyzer to measure the CO₂ concentration inside the incubator. Access the CO₂ calibration menu in the incubator’s service settings. Follow the on-screen instructions to perform a zero-span calibration, which involves setting a zero reference point and a span (usually using a known concentration of CO₂ gas).
- Humidity Calibration:
Measure the relative humidity using a calibrated hygrometer. Adjust the incubator’s humidity settings to align with the reference measurement.
- Validation: After calibration, allow the incubator to stabilize and re-measure the parameters to confirm accuracy.
The frequency of calibration can vary based on usage and manufacturer recommendations. Generally, it’s advisable to calibrate CO₂ incubators at least once a year. However, in high-usage scenarios or critical applications, more frequent calibration, such as quarterly or biannually, may be necessary. Regular maintenance checks, including calibration, are recommended to ensure optimal performance.
International Process Solutions (IPS)
International Process Solutions (IPS) is a leading provider of calibration services, specializing in GMP calibration regulatory maintenance. They offer ISO-accredited calibration services across various disciplines, including temperature, pressure, humidity, and CO₂ levels, ensuring that laboratory equipment like CO₂ incubators operate within specified parameters.
IPS US
IPS provides both on-site and laboratory calibration services, catering to the specific needs of pharmaceutical and biotech industries. Their team is trained in cGMP (current Good Manufacturing Practice) to ensure compliance with regulatory standards. With multiple lab locations in California, including San Carlos, San Diego, and Orange County, IPS is well-positioned to serve clients across the region.
Regular calibration of CO₂ incubators is essential for maintaining experimental accuracy, regulatory compliance, and equipment longevity. Partnering with experienced calibration service providers like International Process Solutions can ensure that your equipment remains in optimal condition, supporting the integrity of your laboratory work.